作者
Erin L Connolly, Janette P Fett, Mary Lou Guerinot
发表日期
2002/6/1
期刊
The Plant Cell
卷号
14
期号
6
页码范围
1347-1357
出版商
American Society of Plant Biologists
简介
Iron, an essential nutrient, is not readily available to plants because of its low solubility. In addition, iron is toxic in excess, catalyzing the formation of hydroxyl radicals that can damage cellular constituents. Consequently, plants must carefully regulate iron uptake so that iron homeostasis is maintained. The Arabidopsis IRT1 gene is the major transporter responsible for high-affinity iron uptake from the soil. Here, we show that the steady state level of IRT1 mRNA was induced within 24 h after transfer of plants to iron-deficient conditions, with protein levels peaking 72 h after transfer. IRT1 mRNA and protein were undetectable 12 h after plants were shifted back to iron-sufficient conditions. Overexpression of IRT1 did not confer dominant gain-of-function enhancement of metal uptake. Analysis of 35S-IRT1 transgenic plants revealed that although IRT1 mRNA was expressed constitutively in these plants, IRT1 protein …
引用总数
学术搜索中的文章
EL Connolly, JP Fett, ML Guerinot - The Plant Cell, 2002