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Monitoring internal training load has been extensively used and described within team sport environments, however when compared to internal physiological measures such as heart rate (HR) and rating of perceived exertion (RPE), the literature is sparse. The primary aim of this investigation study was to assess differences of playing position on ITL, session-RPE and wellness across two different training microcycles (1 vs. 2 competitive games), in addition with examining the relationship between ITL and Hooper's Index across an entire season. Thirty-five professional soccer players from the Portuguese premier league participated in the study (25.7 ± 5.0 years; 182.3 ± 6.4 cm; 79.1 ± 7.0 kg). Analysis of variance revealed higher values of DOMS (Means(M): 3.33 vs. 3.10; p = 0.001; effect Size (ES) = 0.087), fatigue (M: 3.18 vs. 2.99; p = 0.001; ES = 0.060) and HI (M: 11.85 vs. 11.56; p = 0.045; ES = 0.034) in 2-game …