作者
Imran Bhatti, Andrew Lee, Victoria James, Richard I Hall, Jonathan N Lund, Cristina Tufarelli, Dileep N Lobo, Michael Larvin
发表日期
2011/1/1
期刊
Journal of Gastrointestinal Surgery
卷号
15
期号
1
页码范围
199-208
出版商
No longer published by Elsevier
简介
Objective
This study aims to examine the expression of a panel of five microRNAs (miRNA) in pancreatic ductal adenocarcinoma (PDAC) and the functional effect of miR-21 inhibition in PDAC cell lines.
Background
miRNA are short, non-coding RNA molecules, which play important roles in several cellular processes by silencing expression of their target genes through translational repression or mRNA degradation. They are often aberrantly expressed in cancer, and this dysregulation can promote carcinogenesis by altering the expression of tumour suppressor or oncogenes.
Methods
miRNA expression levels were measured in 24 PDAC tumour/matched adjacent normal tissue samples and three PDAC cell lines using reverse transcription polymerase chain reaction. Levels of cell proliferation and death and expression of programmed cell death 4 (PDCD4; tumour suppressor) were studied in PDAC cells (MIA-Pa-Ca …
学术搜索中的文章
I Bhatti, A Lee, V James, RI Hall, JN Lund, C Tufarelli… - Journal of Gastrointestinal Surgery, 2011