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In higher plants, acis-acting element, DRE/CRT, is involved in gene expression responsive to drought and low-temperature stress. To understand signal transduction pathways from the cold stress signal to gene expression, we characterized a gene family for DRE/CRT-binding proteins DREB1A and CBF1 inArabidopsis thaliana.DREB1A and CBF1 were shown to be involved in low-temperature-responsive gene expression. We screened anArabidopsisgenomic DNA library with the cDNA fragment of DREB1A as a probe and isolatedDREB1Aand 2 related genes,DREB1B(= CBF1) andDREB1C.These were arrayed in the order B, A, C in an 8.7 kb region ofArabidopsischromosome 4. Northern blot analysis using gene-specific probes showed that the 3DREB1genes are induced mainly by cold stress but not by osmotic stress in leaves, roots, and stems. Several conserved sequences were found in the promoter …