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Gelsolin is an actin‐severing protein whose action is initiated by Ca²⁺ and inhibited by binding to phosphorylated inositol lipid or phosphoinositides. The regions of gelsolin responsible for phosphoinositide binding are comprised of residues 150–169 (G150–169) and 135–142 (G135–142). The corresponding peptides possess similar binding potency as native gelsolin. Their common feature is the presence of arginine and lysine residues that can bind to negatively charged phosphate groups of phosphoinositides. In this work the binding of the G150–169 peptide to a phosphatidylinositol 4,5‐bisphosphate (PIP2) cluster in a lipid membrane model was investigated by molecular dynamics calculations (MD) with the AMBER 4.1 force field, taking into account explicit solvent molecules. Initially the structure of G150–169 was simulated by using the electrostatically driven Monte Carlo (EDMC) and MD methods, and the …