作者
Shiqi Zhou, OA Karl, Moustafa T Mabrouk, Dushyant Jahagirdar, Wei-Chiao Huang, Julio A Guerra, Xuedan He, Joaquin Ortega, Steven T Poole, Eric R Hall, Oscar G Gomez-Duarte, Milton Maciel Jr, Jonathan F Lovell
发表日期
2023/12/1
期刊
Biomedical Journal
卷号
46
期号
6
页码范围
100588
出版商
Elsevier
简介
Background
Enterotoxigenic Escherichia coli (ETEC) strains cause infectious diarrhea and colonize host intestine epithelia via surface-expressed colonization factors. Colonization factor antigen I (CFA/I), a prevalent ETEC colonization factor, is a vaccine target since antibodies directed to this fimbria can block ETEC adherence and prevent diarrhea.
Methods
Two recombinant antigens derived from CFA/I were investigated with a vaccine adjuvant system that displays soluble antigens on the surface of immunogenic liposomes. The first antigen, CfaEB, is a chimeric fusion protein comprising the minor (CfaE) and major (CfaB) subunits of CFA/I. The second, CfaEad, is the adhesin domain of CfaE.
Results
Owing to their His-tag, recombinant CfaEB and CfaEad, spontaneously bound upon admixture with nanoliposomes containing cobalt-porphyrin phospholipid (CoPoP), as well as a synthetic monophosphoryl lipid A …
学术搜索中的文章
S Zhou, OA Karl, MT Mabrouk, D Jahagirdar… - Biomedical Journal, 2023