作者
Chan Zhang, Zejun Jiang, Maojun Jin, Pengfei Du, Ge Chen, Xueyan Cui, Yudan Zhang, Guoxin Qin, Feiyan Yan, AM Abd El-Aty, Ahmet Hacimüftüoğlu, Jing Wang
发表日期
2020/10/1
期刊
Food chemistry
卷号
326
页码范围
126813
出版商
Elsevier
简介
Herein, we developed a multi-analyte fluorescence immunoassay for detection of three organophosphate pesticides (triazophos, parathion, and chlorpyrifos) in various agro-products (rice, wheat, cucumber, cabbage, and apple) using fluorescently labeled oligonucleotide and gold nanoparticle (AuNP) signal amplification technology. The AuNP probes for the three analytes were constructed by simultaneously modifying the corresponding antibodies and fluorescently labeled oligonucleotides on the probe surface. Three fluorophores (6-FAM, Cy3, and Texas red) with high fluorescence intensity and little overlap of excitation/emission wavelengths were selected. The method showed satisfactory linearity for triazophos, parathion, and chlorpyrifos in the ranges of 0.01–20, 0.05–50, and 0.5–1000 μg/L, respectively. For the 3 analytes, the limits of detection (LODs) were 0.007, 0.009, and 0.087 μg/L, respectively. The …
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