作者
Syed J Khundmiri, Vishal Amin, Jeff Henson, John Lewis, Mohamed Ameen, Madhavi J Rane, Nicholas A Delamere
发表日期
2007/9
期刊
American Journal of Physiology-Cell Physiology
卷号
293
期号
3
页码范围
C1171-C1180
出版商
American Physiological Society
简介
Endogenous cardiotonic glycosides bind to the inhibitory binding site of the plasma membrane sodium pump (Na+/K+-ATPase). Plasma levels of endogenous cardiotonic glycosides increase in several disease states, such as essential hypertension and uremia. Low concentrations of ouabain, which do not inhibit Na+/K+-ATPase, induce cell proliferation. The mechanisms of ouabain-mediated response remain unclear. Recently, we demonstrated that in opossum kidney (OK) proximal tubular cells, low concentrations of ouabain induce cell proliferation through phosphorylation of protein kinase B (Akt) in a calcium-dependent manner. In the present study, we identified ERK as an upstream kinase regulating Akt activation in ouabain-stimulated cells. Furthermore, we provide evidence that low concentrations of ouabain stimulate Na+/K+-ATPase-mediated 86Rb uptake in an Akt-, ERK-, and Src kinase-dependent …
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SJ Khundmiri, V Amin, J Henson, J Lewis, M Ameen… - American Journal of Physiology-Cell Physiology, 2007