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Temperament scoring typically occurs at the same time as vaccinations, germplasm collection, or both. Little is known about the impact of these procedures with scoring temperament. The objective was to determine the impact of blood collection (jugular venipuncture), representing a momentarily painful procedure, on different measures of temperament in weaning age calves. The hypothesis is blood collection will have little to no impact on temperament assessment. Calves of weaning age (n == 420) were sired by Angus bulls and out of Angus-influenced commercial cows. Subjective methods included temperament score (1 to 5 scale, with 3 removed; 1 calm or docile and 5 wild or aggressive) and qualitative behavior assessment attributes of active, agitated, apathetic, attentive, calm, curious, distressed, fearful, happy, irritated, positively occupied, and relaxed (each scored on a 136 mm horizontal line with left …