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A single exposure to menthol can, depending on concentration, enhance both cool sensations and encourage body heat storage. This study tested whether there is an habituation in either response after repeated-daily exposures. Twenty-two participants were assigned to one of three spray groups: Control (CON; n = 6), 0.05% l-menthol (M_0.05%; n = 8), and 0.2% l-menthol (M_0.2%; n = 8). On Monday (20 °C, 50% rh) participants were sprayed with 100 mL of solution and undertook 40 min of cycling at 45% of their peak power (Ex₁), from Tuesday to Thursday (30 °C, 50% rh) they were sprayed twice daily whilst resting (R₁ to R₆), Friday was a repeat of Monday (Ex₂). Thermal sensation (TS), thermal comfort, perceived exertion, irritation, rectal and skin temperature (T_sk), skin blood flow (SkBF) and sweat rate were monitored. A two-way ANOVA (alpha = 0.05) compared responses from the beginning (Ex₁, R₁) and …