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Soybean (Glycine max L.) seedlings were grown in a hydroponic system containing nutrient solution with varying concentrations (0, 0.01, 0.5 mM) of cadmium (Cd) for a 48 h period. Roots and leaves were analyzed for catalase, glutathione reductase, and superoxide dismutase activities. Although a clear effect of Cd on plant growth was observed, the activities of catalase and superoxide dismutase were not altered significantly in the roots or leaves at all concentrations tested. Glutathione reductase did not exhibit any variation in activity in leaves, however, in roots an increase in activity was observed at 0.5 mM Cd. Catalase and superoxide dismutase isoenzymes were further characterized by analysis in non-denaturing PAGE. Activity staining for superoxide dismutase (SOD) revealed seven isoenzymes in leaves and eight isoenzymes in roots, corresponding to Mn-SOD and Cu/Zn-SOD. No Fe-SOD isoenzymes …