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The cellulase enzyme is currently the world's third-largest enzyme. Because of their demands in various industries like textiles, food, waste management, pharmaceuticals, agriculture, pulp paper, biofuels, etc., their demand curve shifts upward sharply. The response surface methodology (RSM) approach was employed to optimize media components and process parameters in the current investigation, which successfully increased cellulase production from Penicillium funiculosum NCIM 1228 (P. funiculosum NCIM 1228). Statistical optimization for the hyperproduction of cellulases has been conducted using RSM. The Plackett-Burman design (PBD) approach was used to investigate the critical factors of the cellulase production medium, followed by the Box-Behnken design (BBD) method, which was used to statistically estimate optimum values and conditions that had a substantial impact on cellulase production. The estimated optimal combinations of parameters for cellulase production were urea (0.2,%), CaCl 2 (0.2,%), MgSO 4 (0.05,%), peptone (1.5,%), microcrystalline cellulose (5.0,%), wheat bran (2.5,%), CSL (2.5,%), KH 2 PO 4 (0.15,%), inoculum (10.65%), agitation (157 rpm), pH (5.88), and temperature (29.84 C). Conclusively, experimental validation under optimal conditions detected an increased production of 3.82-fold and 3.61-fold in filter paper activity (FPase) and β-glucosidase respectively while 1.66-fold and 1.57-fold improvement in FPase and β-glucosidase specific activity were observed. The xylanase activity was enhanced by 3.29-fold and its specific activity improved by 69.62% after optimization. P. funiculosum NCIM 1228 …