作者
Robert Matthew Kottmann, Ajit A Kulkarni, Katie A Smolnycki, Elizabeth Lyda, Thinesh Dahanayake, Rami Salibi, Sylvie Honnons, Carolyn Jones, Nancy G Isern, Jian Z Hu, Steven D Nathan, Geraldine Grant, Richard P Phipps, Patricia J Sime
发表日期
2012/10/15
期刊
American journal of respiratory and critical care medicine
卷号
186
期号
8
页码范围
740-751
出版商
American Thoracic Society
简介
Rationale: Idiopathic pulmonary fibrosis (IPF) is a complex disease for which the pathogenesis is poorly understood. In this study, we identified lactic acid as a metabolite that is elevated in the lung tissue of patients with IPF.
Objectives: This study examines the effect of lactic acid on myofibroblast differentiation and pulmonary fibrosis.
Methods: We used metabolomic analysis to examine cellular metabolism in lung tissue from patients with IPF and determined the effects of lactic acid and lactate dehydrogenase-5 (LDH5) overexpression on myofibroblast differentiation and transforming growth factor (TGF)-β activation in vitro.
Measurements and Main Results: Lactic acid concentrations from healthy and IPF lung tissue were determined by nuclear magnetic resonance spectroscopy; α-smooth muscle actin, calponin, and LDH5 expression were assessed by Western blot of cell culture lysates. Lactic acid and LDH5 were …
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RM Kottmann, AA Kulkarni, KA Smolnycki, E Lyda… - American journal of respiratory and critical care …, 2012
