作者
Kate GR Quinlan, Marco Nardini, Alexis Verger, Pierangelo Francescato, Paul Yaswen, Daniela Corda, Martino Bolognesi, Merlin Crossley
发表日期
2006/11/1
期刊
Molecular and cellular biology
卷号
26
期号
21
页码范围
8159-8172
出版商
American Society for Microbiology
简介
Numerous transcription factors recruit C-terminal binding protein (CtBP) corepressors. We show that the large zinc finger protein ZNF217 contacts CtBP. ZNF217 is encoded by an oncogene frequently amplified in tumors. ZNF217 contains a typical Pro-X-Asp-Leu-Ser (PXDLS) motif that binds in CtBP's PXDLS-binding cleft. However, ZNF217 also contains a second motif, Arg-Arg-Thr (RRT), that binds a separate surface on CtBP. The crystal structure of CtBP bound to an RRTGAPPAL peptide shows that it contacts a surface crevice distinct from the PXDLS binding cleft. Interestingly, both PXDLS and RRT motifs are also found in other zinc finger proteins, such as RIZ. Finally, we show that ZNF217 represses several promoters, including one from a known CtBP target gene, and mutations preventing ZNF217's contact with CtBP reduce repression. These results identify a new CtBP interaction motif and establish …
引用总数
学术搜索中的文章
KGR Quinlan, M Nardini, A Verger, P Francescato… - Molecular and cellular biology, 2006