作者
Florian Leuschner, Gabriel Courties, Partha Dutta, Luke J Mortensen, Rostic Gorbatov, Brena Sena, Tatiana I Novobrantseva, Anna Borodovsky, Kevin Fitzgerald, Victor Koteliansky, Yoshiko Iwamoto, Marina Bohlender, Soeren Meyer, Felix Lasitschka, Benjamin Meder, Hugo A Katus, Charles Lin, Peter Libby, Filip K Swirski, Daniel G Anderson, Ralph Weissleder, Matthias Nahrendorf
发表日期
2015/6/14
期刊
European heart journal
卷号
36
期号
23
页码范围
1478-1488
出版商
The University of Chicago Press
简介
Background
Myocarditis is characterized by inflammatory cell infiltration of the heart and subsequent deterioration of cardiac function. Monocytes are the most prominent population of accumulating leucocytes. We investigated whether in vivo administration of nanoparticle-encapsulated siRNA targeting chemokine (C-C motif) receptor 2 (CCR2)—a chemokine receptor crucial for leucocyte migration in humans and mice—reduces inflammation in autoimmune myocarditis.
Methods and results
In myocardium of patients with myocarditis, CCL2 mRNA levels and CCR2+ cells increased (P < 0.05), motivating us to pursue CCR2 silencing. Flow cytometric analysis showed that siRNA silencing of CCR2 (siCCR2) reduced the number of Ly6Chigh monocytes in hearts of mice with acute autoimmune myocarditis by 69% (P < 0.05), corroborated by histological assessment. The …
学术搜索中的文章
F Leuschner, G Courties, P Dutta, LJ Mortensen… - European heart journal, 2015