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Obesity is highly prevalent, and its incidence is increasing. The previous study showing a major effect of paternal obesity on metabolic health of offspring is confounded by comorbidity with diabetes. Therefore, we investigated the effect of diet‐induced paternal obesity, in the absence of diabetes, on the metabolic health of two resultant generations and the molecular profiles of the testes and sperm. Founder (F₀) male C57BL6 mice were fed either a high‐fat diet (HFD) or a control diet (CD); n = 10/diet for a period of 10 wk. Testis expression of mRNA/microRNAs was analyzed by microarray and qPCR and sperm microRNA abundance by qPCR Two subsequent generations were generated by mating F₀ and then F₁ mice to CD mice, and their metabolic health was investigated. All mice, other than F₀ males, were maintained on a CD. HFD feeding induced paternal obesity with a 21% increase in adiposity, but not …