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The aim of the current study was to develop a new screening instrument for lower limb functional assessment. Fifty-three athletes (33M, 20F; mean age: 19.4±2.5 years old) volunteered for the study. Athlete injury history and sport performance level (international, national, regional, recreational) were recorded. A lower limb functional screening tool was developed (the LoLiFST), based on 5 lower limb movements in different modes, planes, directions and at varying intensities. Both legs were assessed in a random order and each athlete was given a technique and a symptom score. Reliability was evaluated. Spearman's correlation was employed to examine the relationship between the measures and the incidence of injury. Receiver operating characteristic (ROC) analysis was employed to assess the instrument's capacity to classify injury status. (1) The test-retest reliability was 0.74. The inter-rater reliability was 0.95.(2) Twenty-five of the athletes had a history of low back or lower limb injures in the past 12 months.(3) Both the technique and symptom scores from the LoLiFST were significantly correlated with the injuries (ρ=-0.290, P= 0.035; ρ=-0.390, P= 0.004), and the two scores were significantly inter-correlated (ρ= 0.354, P= 0.009).(4) When technique or symptom scores alone were included to differentiate between athletes with or without injury, the area under the ROC curve (AUC) scores were 0.668 (P= 0.036, 95% CI: 0.520-0.815) and 0.722 (P= 0.006, 95% CI: 0.582-0.862), respectively. With technique and symptom scores combined, the AUC discrimination score was 0.762 (P= 0.001, 95% CI: 0.634-0.890). When …