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Organizational culture is the combination of the beliefs, values, behavior norms, formal and informal system processes and methods. As of 1980, the organizational culture, which is an important phenomenon in business management, has become a dominant issue in defining the behaviors of the employees in tourism business. Organizational culture in hotel businesses ensure that the employees are motivated towards acting against uncertainties with a mutual purpose, and that they move in a safe path. In this study, the 5-star hotels in İstanbul have been evaluated in terms of organizational culture type (Adhocracy, Klan, Bureaucracy and Market) set by Cameron and Quinn “Competing Values Model”. In the study context, 335 employees replied to questionnaires in 5-star hotels. At the end of the study, it has been determined that the mean values of the organizational culture types are higher; however, there are no clear differences among culture types. Keywords: Organizational Culture, Organizational Culture Types, Hotel Businesses, Competing Values Model