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Determining the employees’ turnover intention in pre-opening hotel businesses is important for the hospitality industry who have to bear the costs of employment and in-service training. This study aims to examine the relation between of the employees who are recruited by opening hotel businesses and their turnover intentions. A questionnaire was utilized on 151 employees who worked at six 5-Star pre-opening hotels in Istanbul. Respondents were selected based on convenience sampling method. According to the results of the study, turnover intentions of the employees in opening hotel businesses are low. It was also found that there is difference on turnover intention based on gender variable.