作者
Shailesh Tripathi, Maneesha S Saxena, Akhilesh K Tyagi, Sube Singh, Deepak Bajaj, Shivali Sharma, Hari D Upadhyaya, Swarup K Parida, CLL Gowda, Shouvik Das, Alice Kujur, Saurabh Badoni
发表日期
2015
出版商
Oxford University Press
简介
Phylogenetic footprinting identified 666 genome-wide paralogous and orthologous CNMS (conserved non-coding microsatellite) markers from 5′-untranslated and regulatory regions (URRs) of 603 protein-coding chickpea genes. The (CT)n and (GA)n CNMS carrying CTRMCAMV35S and GAGA8BKN3 regulatory elements, respectively, are abundant in the chickpea genome. The mapped genic CNMS markers with robust amplification efficiencies (94.7%) detected higher intraspecific polymorphic potential (37.6%) among genotypes, implying their immense utility in chickpea breeding and genetic analyses. Seventeen differentially expressed CNMS marker-associated genes showing strong preferential and seed tissue/developmental stage-specific expression in contrasting genotypes were selected to narrow down the gene targets underlying seed weight quantitative trait loci (QTLs)/eQTLs (expression QTLs …
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