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This study evaluated the potential toxic effects of silver nanoparticles (AgNPs) and silver nanowires (AgNWs) on saltwater microcrustacean Artemia salina nauplii under ISO TS 20787 guideline. To investigate the acute toxicity of these nanomaterials, the nauplii were exposed to different concentrations of 0 (control), 0.39, 1.56, 6.25, 25 and 100 mg/L AgNPs and concentrations of 0 (control), 0.01, 0.1, 1, 10, 50 and 100 mg/L AgNWs for 72 h. Immobilization rate of A. salina exposed to both AgNPs and AgNWs for 72 h increased significantly in a concentration-dependent manner (P < 0.05). The 72 h EC₁₀ and EC₅₀ were found to be 1.48 ± 0.6 and 10.70 ± 1.3 mg/L for AgNPs, respectively, and 0.03 ± 0.02 and 0.43 ± 0.04 mg/L for AgNWs, respectively. Based on the EC₁₀ and EC₅₀ values, the toxicity of AgNWs was significantly higher than AgNPs (P < 0.05). Oxidative stress resulted from 48 h …