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This study aim to evaluate the potential toxic effects of citrate coated silver nanoparticles (AgNPs) and ionic silver (AgNO₃) on marine microalgae Dunaliella salina under three different salinities (35, 70, and 140 g/L). The toxicity was investigated according to modified OECD guideline (No. 201) by 72 h exposure of microalgae to various concentrations of each of the chemicals in Walne's saline media. According to the results, the growth inhibitory effects of AgNPs and AgNO₃ increased significantly coincidence with increasing time and concentration compared to control (P < 0.05). The values of median inhibitory concentrations (IC₅₀) of AgNPs and AgNO₃ based on average specific growth rate and yield for D. salina increased significantly with elevation of water salinity from 35 to 140 g/L (P < 0.05). Toxicity of AgNO₃ based on IC₅₀ to D. salina was significantly higher than AgNPs at all salinities (P < 0.05 …