作者
Antonio Parra, Peiqi Sun, Mariana Graner, Lucas Itto, Jesper Kers, Mariana Veras, Sandrine Florquin, Joris Roelofs, Lucia Andrade, Alessandra Tammaro, Talita Sanches
发表日期
2024/4/1
期刊
Kidney International Reports
卷号
9
期号
4
页码范围
S21-S22
出版商
Elsevier
简介
Methods
In temperature-/humidity-controlled chambers within an ambient particle concentrator, mice were exposed to a concentrated PM2. 5 stream or to high-efficiency particulate air-filtered clean air (CA). Mass concentrations of PM were measured with an airborne particulate monitor, and the target dose was 600 μg m− 3/day (equivalent to the daily exposure in São Paulo). After 12 weeks, some mice underwent bilateral 30-min clamping of the kidney hila and subsequent reperfusion. Mice were divided into 4 groups: CA; PM2. 5; CA+ I/R; and PM2. 5+ I/R. All studies were performed 48 h after I/R.
Results
Renal chemokines (Cxcl1, Ccl2, and Cxcl2) and cytokine Il1b showed increased gene expression in the PM2. 5+ I/R group, in which there was also significant activation of the cGAS-STING signaling pathway, including phosphorylation of interferon regulatory factor 3. The transcription of these factors was …
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A Parra, P Sun, M Graner, L Itto, J Kers, M Veras… - Kidney International Reports, 2024