作者
R Ragone, F Facchiano, A Facchiano, AM Facchiano, G Colonna
发表日期
1989/5/1
期刊
Protein Engineering, Design and Selection
卷号
2
期号
7
页码范围
497-504
出版商
Oxford University Press
简介
The flexibility plot of a protein lies on the observation that amino acid residues with the highest turn potential, i.e. located in highly mobile regions of protein surface, also possess the smallest volumes as well as the lowest hydrophobicities. The plot is generated by shifting a five residue window along the protein sequence and calculating the value of the hydrophobicity–volume product for consecutive quintuplets of amino acid residues. The concomitant occurrence of small volumes and low hydrophobicities results in very deep minima. A threshold value has also been introduced in order to discriminate significant minima. To substantiate the interpretation that the selected minima actually indicate very flexible segments of a protein (loops, turns, etc.), we have compared plots obtained for model proteins (lysozyme, myoglobin, ribonuclease, trypsin, thermolysin and T4 lysozyme) with X-ray thermal factors profiles …
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R Ragone, F Facchiano, A Facchiano, AM Facchiano… - Protein Engineering, Design and Selection, 1989