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The aim of this study was to isolate and characterize the bioactive compound of Micromonospora auratinigra, HK‐10 and its antibacterial inhibitory mechanism.

An oily bioactive compound was extracted from HK‐10 (GenBank accession no. JN381554) and found to have promising antibacterial activity. The compound was characterized as 2‐methylheptylisonicotinate (1) by ¹H, ¹³C NMR and mass spectroscopy. Minimum inhibitory concentration (MIC) of this molecule was tested by micro broth dilution method and was found to be 70, 40, 80, 60, 60 and 50 μg for Staphylococcus aureus, Bacillus subtilis, Proteus vulgaris, Echerichia coli, Pseudomonas aeruginosa and Mycobacterium abscessus respectively. The effects of compound 1 were studied on bacterial membrane structure using scanning electron microscopy. The results indicated a membrane …