作者
Bonnie Chaban, Sebastien Voisin, John Kelly, Susan M Logan, Ken F Jarrell
发表日期
2006/7
期刊
Molecular microbiology
卷号
61
期号
1
页码范围
259-268
出版商
Blackwell Publishing Ltd
简介
N‐linked glycosylation is recognized as an important post‐translational modification across all three domains of life. However, the understanding of the genetic pathways for the assembly and attachment of N‐linked glycans in eukaryotic and bacterial systems far outweighs the knowledge of comparable processes in Archaea. The recent characterization of a novel trisaccharide [β‐ManpNAcA6Thr‐(1‐4)‐β‐GlcpNAc3NAcA‐(1‐3)‐β‐GlcpNAc]N‐linked to asparagine residues in Methanococcus voltae flagellin and S‐layer proteins affords new opportunities to investigate N‐linked glycosylation pathways in Archaea. In this contribution, the insertional inactivation of several candidate genes within the M. voltae genome and their resulting effects on flagellin and S‐layer glycosylation are reported. Two of the candidate genes were shown to have effects on flagellin and S‐layer protein molecular mass and N‐linked glycan …
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