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Analysis of the complete flagellin glycosylation locus of Campylobacter jejuni strain 81–176 revealed a less complex genomic organization than the corresponding region in the genome strain, C. jejuni NCTC 11168. Twenty‐four of the 45 genes found between Cj1293 and Cj1337 in NCTC 11168 are missing in 81–176. Mutation of six new genes, in addition to three previously reported, resulted in a non‐motile phenotype, consistent with a role in synthesis of pseudaminic acid (PseAc) or transfer of PseAc to flagellin. Mutation of Cj1316c or pseA had been shown to result in loss of the acetamidino form of pseudaminic acid (PseAm). Mutation of a second gene also resulted in loss of PseAm, as well as a minor modification that appears to be PseAm extended with N‐acetyl‐glutamic acid. Previously described mutants in C. jejuni 81–176 and Campylobacter coli VC167 that produced flagella lacking PseAm or PseAc …