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Perfluorinated (aliphatic) acids (PFAs) and congeners have many applications in various industrial fields and household for decades. Years later they have been detected in wildlife and this has spurred interest in environmental occurrence as well as influencing living organisms. PFAs were established as peroxisome proliferators and hepatocarcinogens. Amphipatic structure suggests that they may alter cell membrane potential (mbΔΨ) and/or induce changes in cytosolic pH (pHi). The aim of this study was to examine the correlation between changes of above parameters and PFAs structure (CF₆–CF₁₂) in human colon carcinoma HCT116 cells. mbΔΨ and pHi were measured by flow cytometry using fluorescence polarization of the plasma membrane probe 3,3′-dipentyloxacarbocyanine (DiOC₅(3)) and fluorescein diacetate (FDA), respectively. Dose- and time-dependent manner analysis revealed relatively fast …