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In situ hybridization histochemistry (ISHH) was used to study the distribution of various 5-HT₁ receptor messenger RNAs (mRNA) in the mammalian nervous system. Since the cDNAs encoding the different 5-HT₁ receptors, have not been cloned in one single species, brains of the species appropriate for the 5-HT₁ receptor messenger RNA (mRNA) have been used. Thus, 5-HT_1B and 5-HT_1Dα, mRNA were determined in rat and mouse brain, while 5-HT_1E and 5-HT_1F mRNA were studied in human (and monkey) and guinea-pig brain, respectively.

5-HT_1B and 5-HT_1Dα hybridization signals were predominantly present in caudate-putamen and cortical areas; in addition, 5-HT_1B mRNA was also detected in hippocampus, cerebellum and cerebral arteries. In general, the distribution of 5-HT_1B mRNA was characterized by high densities, whereas 5-HT_1Dα mRNA was expressed at very low levels. Comparison of the …