作者
Gary L Norman, Chen‐Yen Yang, Heather P Ostendorff, Zakera Shums, Mark J Lim, Jinjun Wang, Amany Awad, Gideon M Hirschfield, Piotr Milkiewicz, Donald B Bloch, Kenneth J Rothschild, Christopher L Bowlus, Iannis E Adamopoulos, Patrick SC Leung, Harry J Janssen, Angela C Cheung, Catalina Coltescu, M Eric Gershwin
发表日期
2015/2
期刊
Liver International
卷号
35
期号
2
页码范围
642-651
简介
Background & Aims
Using high‐density human recombinant protein microarrays, we identified two potential biomarkers, kelch‐like 12 (KLHL12) and hexokinase‐1 (HK1), in primary biliary cirrhosis (PBC). The objective of this study was to determine the diagnostic value of anti‐KLHL12/HK1 autoantibodies in PBC. Initial discovery used sera from 22 patients with PBC and 62 non‐PBC controls. KLHL12 and HK1 proteins were then analysed for immunoglobulin reactivity by immunoblot and enzyme‐linked immunosorbent assay (ELISA) in two independent cohorts of PBC and disease/healthy control patients.
Methods
Serum samples from 100 patients with PBC and 165 non‐PBC disease controls were analysed by immunoblot and samples from 366 patients with PBC, 174 disease controls, and 80 healthy donors were tested by ELISA.
Results
Anti‐KLHL12 and anti‐HK1 antibodies were each detected more …
学术搜索中的文章
GL Norman, CY Yang, HP Ostendorff, Z Shums, MJ Lim… - Liver International, 2015