作者
Pedro Aguilar-Garrido, Maria Velasco-Estevez, Miguel Ángel Navarro-Aguadero, María Hernández-Sánchez, Marta Ibáñez-Navarro, Prerna Malaney, Marisa Aitken, Xiaorui Zhang, Adrián Fernández, Alejandra Ortiz-Ruiz, Álvaro Otero-Sobrino, Diego Megías, Manuel Pérez, Jesús Gómez, Gadea Mata, Orlando Domínguez, Osvaldo Graña, Pilar Ximénez, Marta Isasa, Paloma Jimena-de Andrés, Sandra Rodríguez, Raúl Torres, Miguel Lafarga, Oleksandra Sirozh, Vanesa Lafarga, Joaquin Martinez-Lopez, Sean M Post, Miguel Gallardo
发表日期
2023/11/28
期刊
Blood
卷号
142
页码范围
5659
出版商
Content Repository Only!
简介
Background: Protein biogenesis is a complex process involving nucleoli and ribosomes. Alterations in any step could lead to alterations in ribosome functionality and protein synthesis. Hnrnpk is an RNA-binding protein (RBP) involve in these processes, finding that an overexpression (OE) produces nucleus and nucleolar stress (NS), decreases transcription, and drives an imbalance in ribosome biogenesis, causing a reduced translation.
Aims: To elucidate how hnRNP K dysregulation affects the hematopoietic stem cell (HSCs) biology.
Methods: To study the impact of Hnrnpk OE in vivo, we developed an inducible tamoxifen mouse model, Hnrnpk Tg/hUbc-CreERT2. Survival was evaluated by Kaplan-Meier, phenotype was described by symptoms/signs, CBC, bone marrow (BM) H/E, IHC and FCM analysis, and serum IL-6 ELISA.
HSCs were cultured to study the impact of Hnrnpk OE in the HSCs dynamics. Hnrnpk …
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P Aguilar-Garrido, M Velasco-Estevez… - Blood, 2023