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In this study, the Ag/Fe₃O₄ nanocomposite was constructed by the leaf extract of Eryngium planum (L.) as an appropriate antibacterial and cytotoxic agent. The green synthesized nanostructure was characterized using UV–visible, X-ray diffraction analysis (XRD), field emission scanning electron microscope (FESEM), and energy-dispersive X-ray spectroscopy (EDX) and Fourier-transform infrared spectroscopy (FT-IR) techniques. In addition, the antibacterial and antifungal activity of Ag/Fe₃O₄ nanocomposite was determined by the disk diffusion method. The cytotoxicity of Ag/Fe₃O₄ nanocomposite was determined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. XRD and FESEM analyses showed the crystalline nature of Ag/Fe₃O₄ nanocomposite and the spherical shape nanoparticles (NPs) with an average size of 26–42 nm, respectively. The antibacterial results indicated that the …