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Althea kurdica is, a well-known plant drug, widely used in Iran as well as other countries. Althea flowers extract have been used extensively for urinary and respiratory disorders. The cytotoxic effects of A. kurdica flowers extracts were evaluated using Human Leukemia Cell Lines (line k562) and human lymphocytes. The cytotoxic activity was determined by anti-proliferative effects on the human lymphocytes and human bone marrow cell line were cultured as a model of normal and cancerous cells respectively under different concentrations. After 24 h treatment with the extracts, cell proliferation was measured using a colorimetric method based on the ability of metabolic active cells to cleave the yellow tetrazolium salt MTT to an insoluble purple formazan crystal. The soluble formazan dye was directly quantified using a scanning multiwall spectrophotometer (ELISA plate reader). A dose response assay with the extracts was carried out to determine the maximum effective dilution. The results showed that the extract exhibited a potent cytotoxic activity against the k562 cell lines and human lymphocytes in specific manner with an IC50 value of 7.5 mg and 5 mg respectively. The traditional extract of A. kurdica roots showed in vitro cytotoxic effects on two different cell lines. Interestingly the IC50 value was higher in the normal human lymphocytes comparing to the Human Bone Marrow Cells. Thus, the concentrations between 5 mg and 7.5 mg could be used for cancer treatment.