作者
Zudin A Puthucheary, Ronan Astin, Mark JW Mcphail, Saima Saeed, Yasmin Pasha, Danielle E Bear, Despina Constantin, Cristiana Velloso, Sean Manning, Lori Calvert, Mervyn Singer, Rachel L Batterham, Maria Gomez-Romero, Elaine Holmes, Michael C Steiner, Philip J Atherton, Paul Greenhaff, Lindsay M Edwards, Kenneth Smith, Stephen D Harridge, Nicholas Hart, Hugh E Montgomery
发表日期
2018/10/1
期刊
Thorax
卷号
73
期号
10
页码范围
926-935
出版商
BMJ Publishing Group Ltd
简介
Objectives
To characterise the sketetal muscle metabolic phenotype during early critical illness.
Methods
Vastus lateralis muscle biopsies and serum samples (days 1 and 7) were obtained from 63 intensive care patients (59% male, 54.7±18.0 years, Acute Physiology and Chronic Health Evaluation II score 23.5±6.5).
Measurements and main results
From day 1 to 7, there was a reduction in mitochondrial beta-oxidation enzyme concentrations, mitochondrial biogenesis markers (PGC1α messenger mRNA expression (−27.4CN (95% CI −123.9 to 14.3); n=23; p=0.025) and mitochondrial DNA copy number (−1859CN (IQR −5557–1325); n=35; p=0.032). Intramuscular ATP content was reduced compared tocompared with controls on day 1 (17.7mmol/kg /dry weight (dw) (95% CI 15.3 to 20.0) vs. 21.7 mmol/kg /dw (95% CI 20.4 to 22.9); p<0.001) and decreased over 7 days (−4.8 mmol/kg dw (IQR −8.0–1.2); n=33; p=0 …
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ZA Puthucheary, R Astin, MJW Mcphail, S Saeed… - Thorax, 2018