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Parasitic nematodes not only cause deadly diseases in plants and animals but also adversely affect agricultural industry and global health, particularly in developing countries. In this study, we planned to combine the concept of enzyme immobilization with nanotechnology to develop magnetic nanoparticles (MNPs) with efficient nematicidal activity in water. A novel nematicidal platform was developed by immobilizing protease (from Streptomyces griseus) on the surface of polymer brush-grafted magnetic nanoparticles (MNPs–PGMA–Pro). For comparison, a monolayer-based nematicidal platform was also developed by functionalizing protease on the surface of glutaraldehyde-functionalized MNPs (MNPs–GA–Pro). MNPs–PGMA–Pro particles show enhanced enzyme activity and stability over a wide range of temperature and pH, as compared to MNPs–GA–Pro. Polymer brush- and monolayer-based protease …