作者
Ching-Lee Wong, Sook-Yee Gan, Siew-Moi Phang
发表日期
2004/12
期刊
Journal of Applied Phycology
卷号
16
页码范围
439-445
出版商
Kluwer Academic Publishers
简介
Two Sargassum species (S. baccularia and S. polycystum) collected from Teluk Kemang and Cape Rachado, Port Dickson, Negeri Sembilan, Malaysia, which are alike in morphology except for the rhizoidal system and vesicles, were characterised using random amplified polymorphism DNA (RAPD). The genomic DNA of both species was isolated from the leaves using a modified CTAB method. Four random primers, that is, OPA2, OPA3, OPA4 and OPA13, successfully amplified the DNA. The polymorphisms generated by these four primers were analysed using the Dice Coefficient of Similarity and cluster analysis was carried out using GelCompar II Version 2.0 (Applied Maths, Kortrijk, Belgium) based on UPGMA. DNA analysis showed that three primers were able to differentiate the two species. Morphological analysis using Principal Components Analysis (PCA) and discriminant function analysis …
学术搜索中的文章
CL Wong, SY Gan, SM Phang - Journal of Applied Phycology, 2004