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Biocrusts are critical components of desert ecosystems, significantly modifying the surfaces they occupy. The mixture of biological components and soil particles that form the crust, in conjunction with moisture, determines the biocrusts’ spectral signatures. Proximal and remote sensing in complementary spectral regions, namely the reflective region, and the thermal region, have been used to study biocrusts in a non-destructive manner, in the laboratory, in the field, and from space. The objectives of this review paper are to present the spectral characteristics of biocrusts across the optical domain, and to discuss significant developments in the application of proximal and remote sensing for biocrust studies in the last few years. The motivation for using proximal and remote sensing in biocrust studies is discussed. Next, the application of reflectance spectroscopy to the study of biocrusts is presented followed by a review …