作者
Fernando H Cincotto, Gonzalo Martínez-García, Paloma Yáñez-Sedeño, Thiago C Canevari, Sergio Antonio Spinola Machado, José M Pingarrón
发表日期
2016/1/15
期刊
Talanta
卷号
147
页码范围
328-334
出版商
Elsevier
简介
This work describes the preparation of an electrochemical immunosensor for ethinylestradiol (EE2) based on grafting of diazonium salt of 4-aminobenzoic acid onto a glassy carbon electrode modified with silver nanoparticles/SiO2/graphene oxide hybrid followed by covalent binding of anti-ethinylestradiol (anti-EE2) to activated carboxyl groups. A competitive immunoassay was developed for the determination of the hormone using peroxidase-labeled ethinylestradiol (HRP-EE2) and measurement of the amperometric response at −200 mV in the presence of hydroquinone (HQ) as redox mediator. The calibration curve for EE2 exhibited a linear range between 0.1 and 50 ng/mL (r2=0.996), with a detection limit of 65 pg/mL. Interference studies with other hormones related with EE2 revealed the practical specificity of the developed method for the analyte. A good reproducibility, with RSD=4.5% (n=10) was also …
学术搜索中的文章
FH Cincotto, G Martínez-García, P Yáñez-Sedeño… - Talanta, 2016