作者
Morgane Sébert, Alexandre Denadai‐Souza, Muriel Quaranta, Claire Racaud‐Sultan, Sophie Chabot, Philippe Lluel, Nicolas Monjotin, Laurent Alric, Guillaume Portier, Sylvain Kirzin, Delphine Bonnet, Audrey Ferrand, Nathalie Vergnolle
发表日期
2018/9
期刊
British journal of pharmacology
卷号
175
期号
18
页码范围
3656-3668
简介
Background and Purpose
Thrombin is massively released upon tissue damage associated with bleeding or chronic inflammation. The effects of this thrombin on tissue regrowth and repair has been scarcely addressed and only in cancer cell lines. Hence, the purpose of the present study was to determine thrombin's pharmacological effects on human intestinal epithelium growth, proliferation and apoptosis, using three‐dimensional cultures of human colon organoids.
Experimental Approach
Crypts were isolated from human colonic resections and cultured for 6 days, forming human colon organoids. Cultured organoids were exposed to 10 and 50 mU·mL−1 of thrombin, in the presence or not of protease‐activated receptor (PAR) antagonists. Organoid morphology, metabolism, proliferation and apoptosis were followed.
Key Results
Thrombin favoured organoid maturation leading to a decreased number of …
学术搜索中的文章
M Sébert, A Denadai‐Souza, M Quaranta… - British Journal of Pharmacology, 2018