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The aim of the study was to assess the reproducibility of pacing strategy, physiological and perceptual responses during simulated 20-km cycling time trials. Seventeen well-trained male cyclists ( = 4.70 ± 0.33 L min⁻¹) completed three 20-km time trials on a Velotron Pro cycle ergometer within a maximum duration of 14 days. During all trials power output, cadence and respiratory exchange were recorded throughout, rating of perceived exertion and affective response were recorded every 2-km and capillary blood was sampled and assayed for the determination of lactate concentration every 4-km. Power output data was assigned to 1-km ‘bins’ and expressed relative to the mean to quantify pacing strategy. Reproducibility of the pacing strategy and the whole trial mean responses was subsequently quantified using typical error (TE) with 90% confidence intervals. The pacing strategy adopted was …