作者
Jesper V Olsen, Boris Macek, Oliver Lange, Alexander Makarov, Stevan Horning, Matthias Mann
发表日期
2007/9
期刊
Nature methods
卷号
4
期号
9
页码范围
709-712
出版商
Nature Publishing Group US
简介
Peptide sequencing is the basis of mass spectrometry–driven proteomics. Here we show that in the linear ion trap–orbitrap mass spectrometer (LTQ Orbitrap) peptide ions can be efficiently fragmented by high-accuracy and full-mass-range tandem mass spectrometry (MS/MS) via higher-energy C-trap dissociation (HCD). Immonium ions generated via HCD pinpoint modifications such as phosphotyrosine with very high confidence. Additionally we show that an added octopole collision cell facilitates de novo sequencing.
引用总数
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JV Olsen, B Macek, O Lange, A Makarov, S Horning… - Nature methods, 2007