作者
Giuseppe Ferrauto, Enza Di Gregorio, Vincent Auboiroux, Manuel Petit, François Berger, Silvio Aime, Hana Lahrech
发表日期
2018/11
期刊
NMR in Biomedicine
卷号
31
期号
11
页码范围
e4005
简介
In glioma, the acidification of the extracellular tumor microenvironment drives proliferation, angiogenesis, immunosuppression, invasion and chemoresistance. Therefore, quantification of glioma extracellular pH (pHe) is of crucial importance. This study is focused on the application of the YbHPDO3A (ytterbium 1,4,7‐triscarboxymethyl‐1,4,7,10‐tetraazacyclododecane) probe for in vivo glioma pHe quantification using chemical exchange saturation transfer (CEST)‐MRI and its correlation with tumor metabolism assessed by immunohistochemistry.
The U87 glioma mouse model was used (n = 18) and MRI performed at 4.7 T. CEST‐MRI of YbHPDO3A solutions at different pH values showed two resolved CEST spectra at 71 ppm and 99 ppm, both sensitive to pH variations, allowing therefore calculation of the ratiometric curve for in vivo pH quantification. In vivo MRI sequences consisted of T2w for tumor localization, T …
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G Ferrauto, E Di Gregorio, V Auboiroux, M Petit… - NMR in Biomedicine, 2018