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ABSTRACT 52 Interest in the measurement of metabolic rates is growing rapidly, due to the relevance of 53 metabolism for understanding organismal physiology, behaviour, evolution and responses to 54 environmental change. The study of metabolism in aquatic animals is undergoing an especially 55 pronounced expansion, with more researchers utilising intermittent-flow respirometry as a research 56 tool than ever before. Aquatic respirometry measures the rate of oxygen uptake as a proxy for 57 metabolic rates, and the intermittent-flow technique has numerous strengths for use with aquatic 58 animals, allowing metabolic rate to be repeatedly estimated on individual animals over several hours 59 or days and during exposure to various conditions or stimuli. There are, however, no published 60 guidelines for the reporting of methodological details when using this method. Here, we provide the 61 first guidelines for reporting intermittent-flow respirometry methods, in the form of a checklist of 62 criteria that we consider to be the minimum required for the interpretation, evaluation and 63 replication of experiments using intermittent-flow respirometry. Furthermore, using a survey of the 64 existing literature, we show that there has been incomplete and inconsistent reporting of methods 65 for intermittent-flow respirometry over the last several decades. Use of the provided checklist of 66 required criteria by researchers when publishing their work should increase consistency of the 67 reporting of methods for studies that use intermittent-flow respirometry. With the steep increase in 68 studies using intermittent-flow respirometry, now is the ideal time …