作者
Sita S Chandrasekaran, Shreeya Agrawal, Alison Fanton, Aditya R Jangid, Bérénice Charrez, Arturo M Escajeda, Sungmin Son, Roger Mcintosh, Huyen Tran, Abdul Bhuiya, María Díaz de León Derby, Neil A Switz, Maxim Armstrong, Andrew R Harris, Noam Prywes, Maria Lukarska, Scott B Biering, Dylan CJ Smock, Amanda Mok, Gavin J Knott, Qi Dang, Erik Van Dis, Eli Dugan, Shin Kim, Tina Y Liu, Erica A Moehle, Katherine Kogut, Brenda Eskenazi, Eva Harris, Sarah A Stanley, Liana F Lareau, Ming X Tan, Daniel A Fletcher, Jennifer A Doudna, David F Savage, Patrick D Hsu
发表日期
2022/8/11
期刊
Nature Biomedical Engineering
页码范围
1-13
出版商
Nature Publishing Group
简介
Rapid nucleic acid testing is central to infectious disease surveillance. Here, we report an assay for rapid COVID-19 testing and its implementation in a prototype microfluidic device. The assay, which we named DISCoVER (for diagnostics with coronavirus enzymatic reporting), involves extraction-free sample lysis via shelf-stable and low-cost reagents, multiplexed isothermal RNA amplification followed by T7 transcription, and Cas13-mediated cleavage of a quenched fluorophore. The device consists of a single-use gravity-driven microfluidic cartridge inserted into a compact instrument for automated running of the assay and readout of fluorescence within 60 min. DISCoVER can detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in saliva with a sensitivity of 40 copies μl–1, and was 94% sensitive and 100% specific when validated (against quantitative PCR) using total RNA extracted from 63 …
引用总数
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