作者
Yonatan H Grad, Marc Lipsitch, Michael Feldgarden, Harindra M Arachchi, Gustavo C Cerqueira, Michael FitzGerald, Paul Godfrey, Brian J Haas, Cheryl I Murphy, Carsten Russ, Sean Sykes, Bruce J Walker, Jennifer R Wortman, Sarah Young, Qiandong Zeng, Amr Abouelleil, James Bochicchio, Sara Chauvin, Timothy DeSmet, Sharvari Gujja, Caryn McCowan, Anna Montmayeur, Scott Steelman, Jakob Frimodt-Møller, Andreas M Petersen, Carsten Struve, Karen A Krogfelt, Edouard Bingen, François-Xavier Weill, Eric S Lander, Chad Nusbaum, Bruce W Birren, Deborah T Hung, William P Hanage
发表日期
2012/2/21
期刊
Proceedings of the national academy of sciences
卷号
109
期号
8
页码范围
3065-3070
出版商
National Academy of Sciences
简介
The degree to which molecular epidemiology reveals information about the sources and transmission patterns of an outbreak depends on the resolution of the technology used and the samples studied. Isolates of Escherichia coli O104:H4 from the outbreak centered in Germany in May–July 2011, and the much smaller outbreak in southwest France in June 2011, were indistinguishable by standard tests. We report a molecular epidemiological analysis using multiplatform whole-genome sequencing and analysis of multiple isolates from the German and French outbreaks. Isolates from the German outbreak showed remarkably little diversity, with only two single nucleotide polymorphisms (SNPs) found in isolates from four individuals. Surprisingly, we found much greater diversity (19 SNPs) in isolates from seven individuals infected in the French outbreak. The German isolates form a clade within the more diverse …
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YH Grad, M Lipsitch, M Feldgarden, HM Arachchi… - Proceedings of the national academy of sciences, 2012