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For recycling agricultural wastes into the production of biofuel, fermentation technology is currently gaining more attention. Bioethanol is a widely used biofuel, however in industries, the fermentation of hemicellulosic feedstock is challenging since fermenting yeasts consume fewer xylose. To enhance the availability of renewable biofuels, Pioneers are keen to develop industrial strains that assimilate xylose along with glucose. In attempt to eliminate the obstacle, xylanolytic yeast with enhanced xylanase production were investigated. A thermotolerant yeast species, Pichia kudriavzevii strain SVMS2019, isolated from the cow's rumen was studied for xylanase production under solid-state fermentation with simultaneous degradation and saccharification of alkali-treated wheat straw biomass for the liberation of xylose sugars and then conversion into bioethanol. After statistical optimization through response surface …