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Metal oxide nanoparticles have been of substantial intrigue with its wide biological applications in the fields of Nano biotechnology. The sizeable surface area to volume proportion, very low thermal conductivity, high strength, heat insulating properties and pliability has made zirconium oxide nanoparticles extremely useful in medical field, especially as dental filling composites and orthopedic implant materials. They may also have notable antimicrobial properties that might be due to this increase in their surface area and also because their distinctive crystal planes. In the present study, FTIR and UV-VIS Spectra were recorded to characterize the commercial yttrium stabilized zirconium oxide nanoparticles. Their particle size, zeta potential and electrophoretic mobility were determined. Their EDX analysis was also carried out. The nanoparticles were dispersed in water and their activity against several MTCC bacterial cultures and isolated skin flora was assessed.