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The Chronic Wasting Disease Surveillance Expense Cap Software (hereafter, “Software”) computes the maximal number of disease surveillance samples that ought to be collected in each county (or other administrative area) the goal of the disease surveillance program is to detect chronic wasting disease (CWD) in white-tailed deer (Odocoileus virginianus). Using an additive approach, the Software pinpoints the combination of samples that should be tested for two collection categories of deer (hunter harvested and non-hunter harvested) in each county (or other administrative area) of the state to ensure sampling of highest risk counties, while keeping the overall cost of the surveillance program within the predetermined budget. Inputs to the additive expense cap algorithm in the Software broadly include the Optimization Matrix (costs, benefits, and starting prevalence of CWD for each collection category and county or other administrative unit) and the Historical Data (previously enacted sampling strategy in each county or other administrative area). Agencies may further parameterize their Software to achieve specific management objectives, including monitoring known infections, searching for new infections, and providing detailed information to the public. The Software includes (redacted) Optimization Matrices and (redacted) Historical Data from Pennsylvania, US, but the software may be adapted for use in other states and provinces.