作者
R Heales, SEC Davies, TE Bates, JB Clark
发表日期
1995/1
期刊
Neurochemical research
卷号
20
页码范围
31-38
出版商
Kluwer Academic Publishers-Plenum Publishers
简介
The effect of depletion of reduced glutathione (GSH) on brain mitochondrial function and N-acetyl aspartate concentration has been investigated. Using pre-weanling rats, GSH was depleted by L-buthionine sulfoximine administration for up to 10 days. In both whole brain homogenates and purified mitochondrial preparations complex IV (cytochrome c oxidase) activity was decreased, by up to 27%, as a result of this treatment. In addition, after 10 days of GSH depletion, citrate synthase activity was significantly reduced, by 18%, in the purified mitochondrial preparations, but not in whole brain homogenates, suggesting increased leakiness of the mitochondrial membrane. The whole brain N-acetyl aspartate concentration was also significantly depleted at this time point, by 11%. It is concluded that brain GSH is important for the maintenance of optimum mitochondrial function and that prolonged depletion leads …
引用总数
学术搜索中的文章
R Heales, SEC Davies, TE Bates, JB Clark - Neurochemical research, 1995