作者
Filipa Abrunhosa, Sílvia Faria, Paula Gomes, Isabel Tomaz, Joao C Pessoa, David Andreu, Margarida Bastos
发表日期
2005/9/15
期刊
The Journal of Physical Chemistry B
卷号
109
期号
36
页码范围
17311-17319
出版商
American Chemical Society
简介
The interaction of two hybrid peptides of cecropin A and melittin [CA(1−8)M(1−18) and CA(1−7)M(2−9)] with liposomes was studied by differential scanning calorimetry (DSC), circular dichroism (CD), and quasi-elastic light scattering (QELS). The study was carried out with large unilamellar vesicles (LUVs) of three different lipid compositions: 1,2-dimyristoil-sn-glycero-3-phosphocholine (DMPC), 1,2-dimyristoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DMPG) and a binary mixture of DMPC/DMPG, in a wide range of peptide-to-lipid (P:L) molar ratios (0 to 1:7). DSC results indicate that, for both peptides, the interaction depends on membrane composition, with very different behavior for zwitterionic and anionic membranes. CD data show that, although the two peptides have different secondary structures in buffer (random coil for CA(1−7)M(2−9) and predominantly β-sheet for CA(1−8)M(1−18)), they both adopt an α …
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F Abrunhosa, S Faria, P Gomes, I Tomaz, JC Pessoa… - The Journal of Physical Chemistry B, 2005